Selection of reference genes for RT-qPCR studies in blood of beluga whales (Delphinapterus leucas).

نویسندگان

  • I-Hua Chen
  • Jiann-Hsiung Wang
  • Shih-Jen Chou
  • Yeong-Huey Wu
  • Tsung-Hsien Li
  • Ming-Yih Leu
  • Wen-Been Chang
  • Wei Cheng Yang
چکیده

Reverse transcription quantitative PCR (RT-qPCR) is used for research in gene expression, and it is vital to choose appropriate housekeeping genes (HKGs) as reference genes to obtain correct results. The purpose of this study is to determine stably expressed HKGs in blood of beluga whales (Delphinapterus leucas) that can be the appropriate reference genes in relative quantification in gene expression research. Sixty blood samples were taken from four beluga whales. Thirteen candidate HKGs (ACTB, B2M, GAPDH, HPRT1, LDHB, PGK1, RPL4, RPL8, RPL18, RPS9, RPS18, TFRC, YWHAZ) were tested using RT-qPCR. The stability values of the HKGs were determined by four different algorithms. Comprehensive analysis of the results revealed that RPL4, PGK1 and ACTB are strongly recommended for use in future RT-qPCR studies in beluga blood samples. This research provides recommendation of reference gene selection, which may contribute to further mRNA relative quantification research in the peripheral blood leukocytes in captive cetaceans. The gene expression assessment of the immune components in blood have the potential to serve as an important approach to evaluating cetacean health influenced by environmental insults.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Hematology and Plasma Chemistry as Indicators of Health and Ecological Status in Beluga Whales, Delphinapterus leucas

The capture of beluga whales, Delphinapterus leucas, for instrumentation or tagging afforded the opportunity to collect blood, which was analyzed to evaluate the animals’ health and gain information on basic physiological systems. Here, we report on hematological and plasma chemical constituents in samples obtained from 183 belugas, 55 of which were handled during attempts to apply tracking ins...

متن کامل

A probe-based qRT-PCR method to profile immunological gene expression in blood of captive beluga whales (Delphinapterus leucas)

Cytokines are fundamental for a functioning immune system, and thus potentially serve as important indicators of animal health. Quantitation of mRNA using quantitative reverse transcription polymerase chain reaction (qRT-PCR) is an established immunological technique. It is particularly suitable for detecting the expression of proteins against which monoclonal antibodies are not available. In t...

متن کامل

Zooarchaeology of a Focal Resource: Dietary Importance of Beluga Whales to the Precontact Mackenzie Inuit

Ethnohistoric records indicate that the economy of early historic Mackenzie Inuit was centred on the summer hunt for beluga whales (Delphinapterus leucas). However, no systematic attempt has been made to quantify the dietary importance of beluga whales to earlier, precontact-period Mackenzie Inuit societies. This issue is addressed herein through analysis of over 2000 beluga bones recovered fro...

متن کامل

Beluga whale (Delphinapterus leucas) vocalizations and call classification from the eastern Beaufort Sea population.

Beluga whales, Delphinapterus leucas, have a graded call system; call types exist on a continuum making classification challenging. A description of vocalizations from the eastern Beaufort Sea beluga population during its spring migration are presented here, using both a non-parametric classification tree analysis (CART), and a Random Forest analysis. Twelve frequency and duration measurements ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • PeerJ

دوره 4  شماره 

صفحات  -

تاریخ انتشار 2016